rHu PD-1-Fc 蛋白|重组人PD-1/PDCD1 蛋白 (Fc标签)：
产品名称：rHu PD-1-Fc 蛋白|重组人PD-1/PDCD1 蛋白 (Fc标签)
别名: PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
表达方式: A DNA sequence encoding the extracellular domain ( Met 1- Gln 167 ) of human PD1 ( NP_005009.2 ) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主: Human Cells
rHu PD-1-Fc 蛋白|重组人 PD-1/PDCD1 蛋白 (Fc标签) 质控指标
纯度: > 97%, as determined by SDS-PAGE SDS-PAGE:
Bio-activity :Measured by its binding ability in a functional ELISA
Immobilized recombinant human PD-L2 at 1ug/ml ( 100ul/well ) can bind human PD1 / Fc chimera with a linear range of 7.8 - 1000 ng/ml
内毒素: < 1.0 EU per 1μg of the protein as determined by the LAL method.
稳定性: Samples are stable for up to twelve months from date of receipt at -70℃
预测N端: Glu 32
分子量: The recombinant human PD1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 390 amino acids and has a calculated molecular mass of 44 KDa. As a result of glycosylation, rhPD1/Fc monomer migrates as an approximately 60-65 KDa protein in SDS-PAGE under reducing conditions.
缓冲液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
rHu PD-1-Fc 蛋白|重组人 PD-1/PDCD1 蛋白 (Fc标签)使用指南
储存方法: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.