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发布日期:2022/8/24 14:40:00

简介:4-硝基苯基-N-乙酰-β-D-氨基葡糖苷用于快速比色测定人尿中 N-乙酰基-β-氨基葡萄糖苷酶的有用底物。

4-硝基苯基-N-乙酰-β-D-氨基葡糖苷物理化学性质:

密度

1.5±0.1 g/cm3

沸点

694.7±55.0 °C at 760 mmHg

熔点

210 - 212ºC

分子式

C14H18N2O8

分子量

342.301

闪点

374.0±31.5 °C

精确质量

342.106323

PSA

154.07

LogP

0

外观性状

白色粉末

蒸汽压

0.0±2.3 mmHg at 25°C

折射率

1.62

储存条件

常温,避光,通风干燥处,密封保存

稳定性

常温常压下稳定

水溶解性

H2O: 5 mg/mL, clear, colorless to very faintly yellow

 

4-硝基苯基-N-乙酰-β-D-氨基葡糖苷详细介绍:

中文名称:

4-硝基苯基-N-乙酰-β-D-氨基葡糖苷

英文名称:

N-[(2s,3r,4r,5s,6r)-4,5-Dihydroxy-6-(Hydroxymethyl)-2-(4-Nitrophenoxy)oxan-3-Yl]ethanamide

CAS号:

3459-18-5

详细描述:

创赛优选商城提供的[Sigma-Aldrich]4-Nitrophenyl N-Acetyl-β-D-Glucosaminide为创赛科技出品,产品稳定,定制速度快,极大地方便了广大科研客户的使用需求。产品包装为500MG,安全运输。

属性:

质量水平:300

测定:≥99% (TLC)

形式:powder

杂质:≤0.05% Free p-nitrophenol

溶解性:water: 5 mg/mL, clear, colorless to very faintly yellow

储存温度:−20°C

SMILES string:CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O

InChI:1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1

InChI key:OMRLTNCLYHKQCK-DHGKCCLASA-N

应用:

N-acetyl-β-D-glucosaminidase (NAG) has been used:

as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)

as a substrate to study chitinase ChiB1 activity

to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells

包装:

100, 250, 500 mg in poly bottle

1 g in poly bottle

生化/生理作用:

4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.

底物:

N-乙酰-氨基葡糖苷酶的发色底物。

查询关键词:3459-18-54-硝基苯基-N-乙酰-β-D-氨基葡糖苷4-Nitrophenyl N-Acetyl-β-D-Glucosaminide,Sigma-Aldrich,上海现货”。

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